Rabbit anti-KRAS Polyclonal Antibody

簡(jiǎn)要描述：Rabbit anti-KRAS Polyclonal Antibody,WB:1:500-1:1000, IF/ICC:1:50-1:200, ELISA:Recommended starting concentration is 1 μg/mL.

產(chǎn)品型號(hào)：abs115469
廠商性質(zhì)：生產(chǎn)廠家
更新時(shí)間：2025-11-05
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品牌	absin	貨號(hào)	abs115469
規(guī)格	50uL	供貨周期	現(xiàn)貨
主要用途	WB:1:500-1:1000, IF/ICC:1:50-1:200	應(yīng)用領(lǐng)域	化工,生物產(chǎn)業(yè),農(nóng)林牧漁,制藥/生物制藥,綜合

Rabbit anti-KRAS Polyclonal Antibody

概述
別名	KRAS抗體;NS; NS3; OES; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; K-Ras 2; 'C-K-RAS; c-Ki-ras; c-Ki-ras2; KRAS,KRAS
宿主	Rabbit
反應(yīng)種屬	Human, Mouse, Rat
應(yīng)用	WB:1:500-1:1000, IF/ICC:1:50-1:200, ELISA:Recommended starting concentration is 1 μg/mL.
分子量	Detects a band of approximately 21kDa (Predicted molecular weight22kDa)
免疫原	A synthetic peptide corresponding to a sequence within amino acids 30-189 of human KRAS (NP_203524.1).
陽(yáng)性對(duì)照	A-549,NIH/3T3,Raji,HeLa,Mouse spleen,Mouse brain,Mouse lung,Rat brain
性能
形式	Liquid
純化方法	Affinity purification
類(lèi)型	Polyclonal Antibody
同種型	IgG
儲(chǔ)存/保存方法	Store at -20℃. Avoid freeze / thaw cycles.
存儲(chǔ)溶液	PBS with 0.05% proclin300,50% glycerol,pH7.3.
標(biāo)記	Unlabeled
修飾	Unmodified
靶標(biāo)
背景說(shuō)明	This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.
細(xì)胞定位	Cell membrane,Cytoplasm,Cytoplasmic side,Lipid-anchor,cytosol
UniProt	P01116

實(shí)驗(yàn)結(jié)果圖

Western blot analysis of various lysates using KRAS Rabbit pAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

Western blot analysis of lysates from C6 cells, using KRAS Rabbit pAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.